pegfp c1 vector (TaKaRa)
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pegfp c1 vector
Pegfp C1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1+vectors/pmCherry-C1+Vector/bio_rxiv__2025__11__26__690680-258-17-19
Average 96 stars, based on 3286 article reviews
Pegfp C1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1+vectors/pmCherry-C1+Vector/bio_rxiv__2025__11__26__690680-258-17-19
Average 96 stars, based on 3286 article reviews
pegfp c1 vector - by Bioz Stars,
2026-09
96/100 stars
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Plasmid Preparation:Article Title: Optogenetic Control of PIP 3 : PIP 3 Is Sufficient to Induce the Actin-Based Active Part of Growth Cones and Is Regulated via Endocytosis Article Snippet: .. To make CAG promoter-controlled backbone plasmids, a CAG promoter was cut out from the adenovirus cosmid vector (TaKaRa) and subcloned into the CMV promoter region of pEGFP-N1 and Article Title: Activated I-BAR IRSp53 clustering controls the formation of VASP-actin–based membrane protrusions Article Snippet: .. Common plasmids used for mammalian cell transfection include the following: (i) IRSp53 (human, full-length; UniProt, no. Q9UQB8) cloned into pmCherry-N1 and pEGFP-N1 vectors (Clontech), fusing the fluorescent proteins to the C-terminus of IRSp53 (IRSp53-mCherry and IRSp53-eGFP); (ii) IRSp53’s I-BAR domain (mouse, residues 1 to 250; UniProt, no. Q8BKX1) cloned into a pEGFP-N1 vector (I-BAR–eGFP); (iii) Fascin1 (human, full-length; UniProt, no. Q16658) cloned into pmCherry-C1 and Article Title: Paralichthys olivaceus MLKL-mediated necroptosis is activated by RIPK1/3 and involved in anti-microbial immunity Article Snippet: Transient transfection of plasmid into HEK293T cells was performed using Polyjet Transfection Reagent (Signagen, USA). .. For gene overexpression experiments, pmCherry-N1 vectors (Clontech, Mountain View, CA, USA) expressing PoMLKL variants, PoRIPK1, or PoRIPK1ΔCT were constructed, Amplification:Article Title: A single-component optogenetic toolkit for programmable control of microtubule Article Snippet: The plasmid templates for EB1 (#17234), CLIP170 (#54044), CAMSAP1 (# 59036),CAMSAP2 (#59037), KIF5A (#166954), and spastin (#134461) were purchased from Addgene. .. To generate OptoMT, we firstly amplified the PHR domain (residues 1-498) of Arabidopsis thaliana CRY2 (Addgene, #70159) by standard PCR and then inserted the fragment into modified pmCherry-C1 and Polymerase Chain Reaction:Article Title: A single-component optogenetic toolkit for programmable control of microtubule Article Snippet: The plasmid templates for EB1 (#17234), CLIP170 (#54044), CAMSAP1 (# 59036),CAMSAP2 (#59037), KIF5A (#166954), and spastin (#134461) were purchased from Addgene. .. To generate OptoMT, we firstly amplified the PHR domain (residues 1-498) of Arabidopsis thaliana CRY2 (Addgene, #70159) by standard PCR and then inserted the fragment into modified pmCherry-C1 and Modification:Article Title: A single-component optogenetic toolkit for programmable control of microtubule Article Snippet: The plasmid templates for EB1 (#17234), CLIP170 (#54044), CAMSAP1 (# 59036),CAMSAP2 (#59037), KIF5A (#166954), and spastin (#134461) were purchased from Addgene. .. To generate OptoMT, we firstly amplified the PHR domain (residues 1-498) of Arabidopsis thaliana CRY2 (Addgene, #70159) by standard PCR and then inserted the fragment into modified pmCherry-C1 and Binding Assay:Article Title: A single-component optogenetic toolkit for programmable control of microtubule Article Snippet: The plasmid templates for EB1 (#17234), CLIP170 (#54044), CAMSAP1 (# 59036),CAMSAP2 (#59037), KIF5A (#166954), and spastin (#134461) were purchased from Addgene. .. To generate OptoMT, we firstly amplified the PHR domain (residues 1-498) of Arabidopsis thaliana CRY2 (Addgene, #70159) by standard PCR and then inserted the fragment into modified pmCherry-C1 and Derivative Assay:Article Title: A single-component optogenetic toolkit for programmable control of microtubule Article Snippet: The plasmid templates for EB1 (#17234), CLIP170 (#54044), CAMSAP1 (# 59036),CAMSAP2 (#59037), KIF5A (#166954), and spastin (#134461) were purchased from Addgene. .. To generate OptoMT, we firstly amplified the PHR domain (residues 1-498) of Arabidopsis thaliana CRY2 (Addgene, #70159) by standard PCR and then inserted the fragment into modified pmCherry-C1 and Transfection:Article Title: Activated I-BAR IRSp53 clustering controls the formation of VASP-actin–based membrane protrusions Article Snippet: .. Common plasmids used for mammalian cell transfection include the following: (i) IRSp53 (human, full-length; UniProt, no. Q9UQB8) cloned into pmCherry-N1 and pEGFP-N1 vectors (Clontech), fusing the fluorescent proteins to the C-terminus of IRSp53 (IRSp53-mCherry and IRSp53-eGFP); (ii) IRSp53’s I-BAR domain (mouse, residues 1 to 250; UniProt, no. Q8BKX1) cloned into a pEGFP-N1 vector (I-BAR–eGFP); (iii) Fascin1 (human, full-length; UniProt, no. Q16658) cloned into pmCherry-C1 and Clone Assay:Article Title: Activated I-BAR IRSp53 clustering controls the formation of VASP-actin–based membrane protrusions Article Snippet: .. Common plasmids used for mammalian cell transfection include the following: (i) IRSp53 (human, full-length; UniProt, no. Q9UQB8) cloned into pmCherry-N1 and pEGFP-N1 vectors (Clontech), fusing the fluorescent proteins to the C-terminus of IRSp53 (IRSp53-mCherry and IRSp53-eGFP); (ii) IRSp53’s I-BAR domain (mouse, residues 1 to 250; UniProt, no. Q8BKX1) cloned into a pEGFP-N1 vector (I-BAR–eGFP); (iii) Fascin1 (human, full-length; UniProt, no. Q16658) cloned into pmCherry-C1 and Article Title: Large-scale alternative polyadenylation (APA)-wide association studies to identify putative susceptibility genes in human common cancers Article Snippet: .. The 3’ UTR sequences of STAT6, FBXO38 , and LDAH , along with their corresponding mutant sequences, were cloned separately into Expressing:Article Title: Phosphatidylinositol 3-kinase-independent synthesis of PtdIns(3,4)P2 by a phosphotransferase Article Snippet: Plasmids were constructed using In-Fusion HD EcoDry Cloning Kits (Takara 121416), NEB HiFi assembly (New England Biolabs E5520S), or traditional restriction enzyme methods and were verified by Sanger sequencing. .. Mammalian expression vectors are based on the Clontech pEGFP-N1 and Article Title: Paralichthys olivaceus MLKL-mediated necroptosis is activated by RIPK1/3 and involved in anti-microbial immunity Article Snippet: Transient transfection of plasmid into HEK293T cells was performed using Polyjet Transfection Reagent (Signagen, USA). .. For gene overexpression experiments, pmCherry-N1 vectors (Clontech, Mountain View, CA, USA) expressing PoMLKL variants, PoRIPK1, or PoRIPK1ΔCT were constructed, Article Title: Mutations in the kinesin KIF12 promote MASH in humans and mice by disrupting lipogenic enzyme turnover Article Snippet: .. The cDNA was then ligated with pEGFP-N1 and Article Title: Mutations in the kinesin KIF12 promote MASH in humans and mice by disrupting lipogenic enzyme turnover. Article Snippet: .. The cDNA was then ligated with pEGFP-N1 and Over Expression:Article Title: Paralichthys olivaceus MLKL-mediated necroptosis is activated by RIPK1/3 and involved in anti-microbial immunity Article Snippet: Transient transfection of plasmid into HEK293T cells was performed using Polyjet Transfection Reagent (Signagen, USA). .. For gene overexpression experiments, pmCherry-N1 vectors (Clontech, Mountain View, CA, USA) expressing PoMLKL variants, PoRIPK1, or PoRIPK1ΔCT were constructed, Construct:Article Title: Paralichthys olivaceus MLKL-mediated necroptosis is activated by RIPK1/3 and involved in anti-microbial immunity Article Snippet: Transient transfection of plasmid into HEK293T cells was performed using Polyjet Transfection Reagent (Signagen, USA). .. For gene overexpression experiments, pmCherry-N1 vectors (Clontech, Mountain View, CA, USA) expressing PoMLKL variants, PoRIPK1, or PoRIPK1ΔCT were constructed, Mutagenesis:Article Title: Large-scale alternative polyadenylation (APA)-wide association studies to identify putative susceptibility genes in human common cancers Article Snippet: .. The 3’ UTR sequences of STAT6, FBXO38 , and LDAH , along with their corresponding mutant sequences, were cloned separately into |